CloningE.coligenes by oligonucleotide hybridization

Oligonucleotide hybridization studied by a surface plasmon diffraction sensor (SPDS).

A novel label-free biosensor concept based on surface plasmon-enhanced diffraction by micro- patterned interfaces was applied to the study of hybridization reactions of target DNA oligonucleotides (15mers and 75mers) from solution to probe DNA oligonucleotides attached via streptavidin to the sensor surface. The self-referencing and quadratic signal amplification mechanism of the sensor allowed...

Detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization

BACKGROUND Simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses. OBJECTIVE The aim of this study was to evaluate the use of 16S rDNA and 23S rDNA sequences as targets for simultaneous detection of eight foodborne bacterial pathogens. METHODS Nineteen bacterial oligonucleotide probes were synthesized and applied to ...

Analysis of nucleic acids by tandem hybridization on oligonucleotide microarrays.

Genotyping male-specific RNA coliphages by hybridization with oligonucleotide probes.

F-specific (F+) RNA coliphages are prevalent in sewage and other fecal wastes of humans and animals. There are four antigenically distinct serogroups of F+ RNA coliphages, and those predominating in humans (groups II and III) differ from those predominating in animals (groups I and IV). Hence, it may be possible to distinguish between human and animal wastes by serotyping F+ RNA coliphage isola...

Enhanced oligonucleotide microarray labeling and hybridization.

Benchmarks Microarrays are a powerful tool for functional studies at the molecular level (1,2). Depending on the experiment, users have a variety of commercial and homemade choices. Most commercially available microarrays come with protocols already optimized for repro-ducibility and sensitivity. However, despite continued reductions in the cost of microarrays, commercially available labeling k...